Onion root tip experiment procedure

Procedure Of The Experiment

1. Place an onion on a tile.
2. With the help of a sharp blade, carefully snip the dry roots of the onion.
3. Place the bulbs in a beaker containing water to grow the root tips.
4. It may take around 4 to 6 days for the new roots to grow and appear.

1. What is the procedure to observing cells in root tip?
2. Why we use onion root tip for experiment?
3. What is the conclusion for the onion root tip experiment?
4. What procedure you will follow to observe stages of mitosis in onion root tip?
5. How do you observe plant cells under a microscope?
6. What is the procedure of mitosis?
7. What is the hypothesis of onion root tip?
8. What is the main function of root tip?
9. What is the purpose of onion diagram?
10. What is the observation of onion cell experiment?
11. What can you conclude about onion cell under microscope?
12. What is the conclusion of onion peel experiment?
13. How would you prepare a root tip squash to observe the stages of mitosis?
14. How do you stain and observe mitosis in garlic root tips?
15. Why are root tips used to observe mitosis?
16. Why is garlic root tip used to observe mitosis?
17. How do onion root tips grow in mitosis?
18. How to prepare onion root tip squash?
19. How are onion root tip slides prepared in order to view them?
20. Which stain used during staining of mitosis in onion root tips?
21. Which stain is used in onion root tip?
22. Which stain is used to observe onion root cells?

What is the procedure to observing cells in root tip?

Cover the root tip with a cover slip and then carefully push down on the cover slide with the wooden end of a dissecting probe. (Push hard, but do not twist or push the cover slide sideways). The root tip should spread out to a diameter of about 0.5- 1cm. Observe it under a compound microscope in 10x objective.

Why we use onion root tip for experiment?

Onion root-tip cells have a cell cycle of approximately 24-hour duration, i.e., they divide once in 24 hours, and this division usually takes place about two hours after sunrise. Therefore, roots grown on water should be cut only at that time to score maximum number of dividing cells.

What is the conclusion for the onion root tip experiment?

The conclusion of this lab was that Mitosis is essential for the production of new cells. In the case of the onion root sample, the cells were damaged leading to the tester to undergo Mitotic cell division and it was found that mainly Interphase and Prophase were the stages that occurred in this lab.

What procedure you will follow to observe stages of mitosis in onion root tip?

Real Lab Procedure:

New roots may take 3–6 days to grow. Cut off 2–3 cm of freshly grown roots and let them drop into a watch glass. Using a forceps, transfer them to the vial containing freshly prepared fixative of aceto-alcohol (1:3: glacial acetic acid: ethanol). Keep the root tips in the fixative for 24 hours.

How do you observe plant cells under a microscope?

Place the glass slide onto the stage. Be careful pushing it under the clips that the cover slide doesn't move or crack. Turn the coarse focus knob slowly until you are able to see the cells. Turn the fine focus knob slowly until the cells are in focus and you can see them clearly.

What is the procedure of mitosis?

Mitosis is conventionally divided into five stages known as prophase, prometaphase, metaphase, anaphase, and telophase. While mitosis is taking place, there is no cell growth and all of the cellular energy is focused on cell division. During prophase, the replicated pairs of chromosomes condense and compact themselves.

What is the hypothesis of onion root tip?

Hypotheses: The experimental hypothesis is that in root tips slices that have been treated with nocodazole, a chemical that interferes with microtubular polymerization, all of the cells will be arrested at the same stage of the cell cycle and that in untreated onion tip slices all of the different stages of the cell ...

What is the main function of root tip?

Darwin realized that the root tip functions as a sensory organ, and that its role is of paramount importance not only for root growth but also for growth and development of the entire plant.

What is the purpose of onion diagram?

Onion diagrams can help you understand the dependencies, relationships, and hierarchies within your organization. You can use them as part of your product development process, to show hierarchies within your organization, or to show the relationships between stakeholders and project goals.

What is the observation of onion cell experiment?

Observations. There are a large number of regularly shaped cells lying side by side and each cell has a distinct cell wall. A distinct nucleus is present on the periphery of each cell. Lightly stained cytoplasm is observed in each cell.

What can you conclude about onion cell under microscope?

Onion skin cells seen through the microscope are also transparent. However, they are generally rectangular in shape, with a distinct cell wall. Like all cells, there is also a plasma membrane surrounding the cell contents, but it clings closely to the cell wall and is hard to see with a light microscope.

What is the conclusion of onion peel experiment?

Conclusion:As cell walls and large vacuoles are clearly observed in all the cells, the cells placed for observation are plant cells. - Onion epidermal peel is made up of rectangular shaped cells. A nucleus, a central vacuole, a thin layer of cytoplasm, and a cell wall make up each cell.

How would you prepare a root tip squash to observe the stages of mitosis?

Gently break up (macerate) the root tip with a mounted needle. Add 1 small drop of orcein ethanoic stain and leave for 2 minutes. Add a coverslip and blot with filter paper. View under a microscope and identify the stages of mitosis.

How do you stain and observe mitosis in garlic root tips?

To see mitosis in action you need to look at living cells. Garlic bulbs grow roots that have actively dividing cells in their tips, in a region called the meristem. Each cell has only eight chromosomes, so it is relatively easy to see the chromosomes once they have condensed.

Why are root tips used to observe mitosis?

Onion root tips are commonly used to study mitosis. They are sites of rapid growth, so the cells are dividing rapidly.

Why is garlic root tip used to observe mitosis?

This experiment use the garlic root tips tissue for observing the mitosis process because the stages of development in plant growth can be distinctly observed at this part which is known as meristem. This meristem is actively divided by mitosis. So, every stage of mitosis can be observed clearly.

How do onion root tips grow in mitosis?

An onion root tip is a rapidly growing part of the onion and thus many cells will be in different stages of mitosis. The onion root tips can be prepared and squashed in a way that allows them to be flattened on a microscopic slide, so that the chromosomes of individual cells can be observed easily.

How to prepare onion root tip squash?

Onion root tip slide (squash method)

Use forceps to transfer an onion root tip into the cup of HCl. Let sit for 4 minutes. After 4 minutes, transfer the root into the Carnoy fluid and let sit for 4 minutes. Remove the root from the fluid and place it on a slide.

How are onion root tip slides prepared in order to view them?

In order to examine cells in the tip of an onion root, a thin slice of the root is placed onto a microscope slide and stained so the chromosomes will be visible. The cells you'll be looking at in this activity were photographed with a light microsope and then digitized so you can see them on the computer.

Which stain used during staining of mitosis in onion root tips?

Acetocarmine is a DNA specific stain like feulgen stain, so the super coiled chromosomes during different stages of mitosis present in the onion root tip cells can be visualized perfectly by treating with this stain.

Which stain is used in onion root tip?

According to the question, the stain used in onion root cells is acetocarmine as it stains the nucleus and helps in viewing different stages of mitosis.

Which stain is used to observe onion root cells?

Safranine is used to stain onion peel cells. This makes them easily observable under a microscope.