Squash preparation of onion root tip for mitotic stages

1. How can we squash preparation of onion root tip to understand the stages of mitosis?
2. What is the squashing technique for onion root tip?
3. What is the squash technique for mitosis?
4. How do you preserve onion root tips for mitosis?
5. What is the purpose of squash technique?
6. Why onion root tip is used for mitosis?
7. What is the squash technique for slide preparation?
8. Why do we squash in microbiology?
9. How do you prepare and stain a root tip squash to observe the stages of mitosis?
10. What is squash smear preparation?
11. What is squash technique in cytology?
12. Which material is used for the squash preparation?
13. How to prepare a root tip squash in order to calculate mitotic index?
14. What is the squash technique for slide preparation?
15. How do you prepare a root tip squash so that chromosomes can be seen?
16. Which part in the onion root tip can we determine the mitotic index?

How can we squash preparation of onion root tip to understand the stages of mitosis?

An onion root tip is a rapidly growing part of the onion and thus many cells will be in different stages of mitosis. The onion root tips can be prepared and squashed in a way that allows them to be flattened on a microscopic slide, so that the chromosomes of individual cells can be observed easily.

What is the squashing technique for onion root tip?

Onion root tip slide (squash method)

Use forceps to transfer an onion root tip into the cup of HCl. Let sit for 4 minutes. After 4 minutes, transfer the root into the Carnoy fluid and let sit for 4 minutes. Remove the root from the fluid and place it on a slide.

What is the squash technique for mitosis?

…the development of the so-called squash technique, in which entire cells are pressed flat on a piece of glass and observed through a microscope; the human chromosomes were numbered using this technique.

How do you preserve onion root tips for mitosis?

Keep the root tips in the fixative for 24 hours and then transfer them to 70% ethanol (for preservation and use in future). Onion root-tip cells have a cell cycle of approximately 24-hour duration, i.e., they divide once in 24 hours, and this division usually takes place about two hours after sunrise.

What is the purpose of squash technique?

This squash procedure provides for extending observations to cytological features of megasporocytes, mega- spores, and megagametophytes previously identified in intact ovules. The new method is applied here to the study of ovule development in two unrelated species, Cassia abbreviata Oliver var.

Why onion root tip is used for mitosis?

Why use onion roots for viewing mitosis? The roots are easy to grow in large numbers. cells can be observed. The chromosomes can be stained to make them more easily observable.

What is the squash technique for slide preparation?

Step 1: Expel the sample onto a clean slide near one end, preferably toward the frosted edge. Step 2: Gently place a second slide flat onto the sample at a right angle, forming a cross shape when viewed from above. Step 3: Using a smooth horizontal motion, spread the material along the slide.

Why do we squash in microbiology?

The advantage of squashing is, that it is a fast and easy method to obtain very thin specimen samples.

How do you prepare and stain a root tip squash to observe the stages of mitosis?

Gently break up (macerate) the root tip with a mounted needle. Add 1 small drop of orcein ethanoic stain and leave for 2 minutes. Add a coverslip and blot with filter paper. View under a microscope and identify the stages of mitosis.

What is squash smear preparation?

Squash smear preparation is a simple and rapid methodology for fairly accurate intraoperative diagnosis of CNS space occupying lesions [2-4]. The main goal of intraoperative cytodiagnosis in stereotactic biopsies is to confirm the adequacy of the tissue.

What is squash technique in cytology?

Squash smear cytology is a rapid intraoperative technique for diagnosing spinal lesions, with high specificity and high positive predictive value. It is more effective in diagnosing neoplasms than non-neoplastic lesions.

Which material is used for the squash preparation?

Squash (sometimes known as cordial in British English, dilute in Hiberno English, and diluting juice in Scottish English) is a non-alcoholic beverage with concentrated syrup used in beverage making. It is usually fruit-flavoured, made from fruit juice, water, and sugar or a sugar substitute.

How to prepare a root tip squash in order to calculate mitotic index?

a Cut off 1-2 cm of the root tips. Put in a small volume of ethanoic acid on a watchglass (or other shallow dish) for 10 minutes. b Meanwhile, heat 10-25 cm³ of 1 M hydrochloric acid to 60 °C in a water bath. c Wash the root tips in cold water for 4-5 minutes and dry on filter paper.

What is the squash technique for slide preparation?

Step 1: Expel the sample onto a clean slide near one end, preferably toward the frosted edge. Step 2: Gently place a second slide flat onto the sample at a right angle, forming a cross shape when viewed from above. Step 3: Using a smooth horizontal motion, spread the material along the slide.

How do you prepare a root tip squash so that chromosomes can be seen?

Add a drop of water to the root tip on the slide. Tease the root tip apart with needles (maceration), to spread out the cells a little. Cover with a coverslip. Replace the lid on the vial of stain and return it to the teacher as instructed.

Which part in the onion root tip can we determine the mitotic index?

Mitotic index decreases with increasing distance from root tip. That means gradual decrease in cell division as it moves from the zone of cell division to the zone of cell elongation. The meristematic region in the root tip is the actively growing region and thus the mitotic index is high.